08-09-2019, 01:13 AM
Hi Everyone!
I've decided to take CCPNMR V3 for a whirl and assign a protein instead of with CARA (a transition from Windows to Mac). :)
Following the Backbone assignment tutorial (using my own data) I can get as far to have all peaks picked in 3D and the Backbone assignment module is indeed matching strips but... Instead of moving each strip to the peak position in Proton AND Nitrogen it does not move in the Nitrogen dimension. See the attached screenshots which shows the matches "40-1" to "6" and "42-1" to "50" but there is no change to the Nitrogen position.
I would like to note that loading the tutorial data and following the guide works as expected with the backbone assignment matches moving to the correct nitrogen chemical shift. So I went ahead and checked the peak lists of my data and everything there appears to be correct. :/
Therefore, I assume this is just an issue of my settings, but I'm not sure where to look. This was a clean install of CCPNMR V3 beta 5 that has been updated twice (127 updates first then 5 updates the second time). Also, the gear icons on the Spectra view nor the Backbone Assignment modules appear to have anything pertaining to locking of a dimension.
I hope is just a simple tweak to my settings or maybe I'm doing something seriously wrong? I can always start again from scratch but was wondering if anyone could provide some advice?
Thanks,
Gage
I've decided to take CCPNMR V3 for a whirl and assign a protein instead of with CARA (a transition from Windows to Mac). :)
Following the Backbone assignment tutorial (using my own data) I can get as far to have all peaks picked in 3D and the Backbone assignment module is indeed matching strips but... Instead of moving each strip to the peak position in Proton AND Nitrogen it does not move in the Nitrogen dimension. See the attached screenshots which shows the matches "40-1" to "6" and "42-1" to "50" but there is no change to the Nitrogen position.
I would like to note that loading the tutorial data and following the guide works as expected with the backbone assignment matches moving to the correct nitrogen chemical shift. So I went ahead and checked the peak lists of my data and everything there appears to be correct. :/
Therefore, I assume this is just an issue of my settings, but I'm not sure where to look. This was a clean install of CCPNMR V3 beta 5 that has been updated twice (127 updates first then 5 updates the second time). Also, the gear icons on the Spectra view nor the Backbone Assignment modules appear to have anything pertaining to locking of a dimension.
I hope is just a simple tweak to my settings or maybe I'm doing something seriously wrong? I can always start again from scratch but was wondering if anyone could provide some advice?
Thanks,
Gage