14^th - 16^th August 2003, St Martin's College, Ambleside, The Lake District

Speakers:

Lewis Kay                                NMR Centre, University of Toronto, Canada

Rolf Boelens                             Utrecht University, The Netherlands

Dusan Uhrin                             University of Edinburgh

Steve Homans                          University of Leeds

Nick Soffe                                Dept. of Biochemistry, University of Oxford

James Keeler                            Dept. of Chemistry, University of Cambridge

Arthur Palmer                           Columbia University, New York, USA

Jörn Werner                             Joern Werner, IDC group, University of Oxford

Bill Broadhurst                      Dept. of Biochemistry, University of Cambridge

James Prestegard                      CRC Biomolecular NMR, University of Georgia

Stefan Grzesiek                        Dept. Structural Biology, Univ. of Basel, Switzerland

Martin Blackledge                    Institut de Biologie Structurale, France

Christina Redfield                     Centre for Molecular Sciences, Univ. of Oxford

PROGRAMME

Thursday 14 August 2003

12.00   Check-in

12.30   Registration and Buffet Lunch

 1.50    Ernest Laue      Welcome and Introduction

Pulse sequences and experimental strategy for assignment

(Chun-Wa Chung  -  Chair)

 2.00    Lewis Kay                    "Tricks with methyls, assignment and beyond........"
 2.30    Rolf Boelens                 "Multidimensional NMR - Resonance assignments, secondary

 structure analysis and structure determination of uniformly

 13C/15N labelled proteins. Application to HU from Bacillus

 stearothermophilus."

 3.00    Dusan Uhrin                 "Protein NMR spectroscopy- practicalities of setting up

 experiments"

 3.30    Tea

 4.00    Steve Homans              "Isotopic enrichment studies for structural and dynamic studies

 of larger proteins."

 4.30    Nick Soffe                    "Probes and Cryoprobes for High-Resolution NMR:

 Design techniques, Problems and Advances."

 5.00    Discussion

 6.00    Poster Session              Drinks provided by Spectra Stable Isotope Group

7.30     Supper

Friday 15 August

Pulse sequences and experimental strategy for relaxation studies

(Paul Driscoll  -  Chair)

 9.15    James Keeler                "What is relaxation and what causes it?"

9.45     Arthur G Palmer           "Experimental methods for spin relaxation studies of protein

 dynamics."

10.15   Jörn Werner                 "Methods to study orientation order in multi-domain proteins."
10.45   Coffee

11.15   Bill Broadhurst  "Anisotropic rotational diffusion."

11.45   Discussion

12.45   Lunch

 2.30    WALK

6.00     Poster Session

 7.00    Bar

 7.30    Conference Dinner

Poster Prize presentation:  Winner - Victoria Higman (University of Oxford)

Saturday 16 August

Pulse sequences and experimental strategy for measurement and use of residual Dipolar couplings (Brian Smith  -  Chair)

 9.15    James Prestegard          "Assembly of Molecular Fragments Using RDC Derived

 Orientations"

 9.45    Stefan Grzesiek            "Biomolecular function, dynamics and folding by RDC and other

 NMR techniques."

10.15   Martin Blackledge        "The use of Residual Dipolar Couplings for Biomolecular

 Structure Determination."

10.45   Coffee

11.15   Christina Redfield         "The use of lanthanides with bicelles for the measurement of

 backbone and side-chain residual dipolar couplings."

11.45   Discussion

 1.00    Lunch

Conference ends

 2.30    WALK